Mark Robins has made three changes to the side that lost to Accrington Stanley on Saturday.
Lee Burge returns to the starting XI after missing the weekend's defeat through injury.
Dom Hyam comes in for Dion Kelly-Evans, pushing Jordan Willis to right-back in a defence that also features Rod McDonald and Chris Stokes.
Peter Vincenti replaces Jordan Maguire-Drew on the right side of midfield with Tom Bayliss, Jordan Shipely and Liam Kelly all keeping their places.
Max Biamou and Marc McNulty start upfront. Former Colchester loanee Kyel Reid is on the bench.
Coventry City XI: Burge (GK), Willis (C), Hyam, McDonald, Stokes, Vincenti, Bayliss, Doyle, Shipley, Biamou, McNulty.
Subs: O'Brien (GK), Haynes, Dev. Kelly-Evans, Barrett, Reid, Clarke-Harris, Ponticelli.
Colchester XI: Walker (GK), Prosser, Kent, Eastman, Wright, Comley, Stevenson, Vincent-Young, Senior, Mandron, Ogedi-Uzokwe.
Subs: Gilmartin (GK), Inniss, Lapslie, Murray, Dickenson, Szmodics, Mandeville.